FastQCFastQC Report
Wed 3 Dec 2025
38392-8_R2.clean.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename38392-8_R2.clean.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36536
Sequences flagged as poor quality0
Sequence length40-150
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGGCGGCTTCCCAACAAGGAGCTGAGCAGGGAGCTGAGCAAGGAGCTCA370.10126998029340924No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTCCG252.7741952E-4117.83687140-144
AGCGTTT250.001732049974.414791
TGTCACT250.001732049974.414793
GGTTCCA300.003568644262.0123332
TCCAGAC450.003863775460.723003125-129
CCCCTTT350.00656914953.1534277
ACTGATG350.00656914953.1534277
TCTCTGA350.00656914953.1534279
CACTGAT350.00656914953.1534276
TCCTACC350.00656914953.1534277
GGGTTCC350.00656914953.1534271
CAAATGG200.001895354236.759235125-129
TGTACTG250.00871561226.915792105-109
TCACCCT300.00956556126.403616140-144
TCCCAGT750.002937368121.479954135-139
ATATTTT500.00726882418.379618125-129
Produced by FastQC (version 0.11.3)