FastQCFastQC Report
Wed 12 Nov 2025
77528-16_R1.clean.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename77528-16_R1.clean.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5379972
Sequences flagged as poor quality0
Sequence length40-150
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTATAC9500.0097619096.6134472
TAGATCG7055.6232027E-56.5162916140-144
GACTTAG13650.00130358865.9835956
ACACGTC26600.05.2871857140-144
Produced by FastQC (version 0.11.3)