FastQCFastQC Report
Wed 3 Dec 2025
81948-8_R1.clean.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename81948-8_R1.clean.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3685309
Sequences flagged as poor quality0
Sequence length40-150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTACGT11850.013.6644535140-144
TACGTCA9550.012.186671140-144
CTACGTC11950.010.586021140-144
ACGTCAA11451.8189894E-1210.16443140-144
TCGTATG5151.964771E-49.825472140-144
CCCTACG14750.09.605648140-144
CTCGTAT6904.056328E-69.533556140-144
CGTCAAA12951.8189894E-129.377825140-144
ACCCTAC18050.08.970846140-144
TATGCCG5250.00254967348.674488140-144
AATCTCG9202.0251537E-68.250193140-144
CACCCTA18652.7284841E-116.783140-144
CGTCTGA16002.7284841E-116.4862256125-129
CGATTCC11001.9254774E-46.42887645
AGTTCGA8800.00539471956.3140755
AGTCACC30000.05.8442283135-139
CTTCGAC13303.4897221E-45.7032561
ACACGTC19107.2759576E-125.683272120-124
AACGGCT10700.0064339625.6644276
TGTAGCG10800.00713027455.6119787
Produced by FastQC (version 0.11.3)